A FRET‐based near‐infrared fluorescent probe for ratiometric detection of cysteine in mitochondria

Authors

Shuai Xia, Michigan Technological University
Yibin Zhang, Michigan Technological UniversityFollow
Mingxi Fang, Michigan Technological UniversityFollow
Logan Mikesell, Michigan Technological University
Tessa Steenwinkel, Michigan Technological UniversityFollow
Shulin Wan, Michigan Technological University
Tyler Phillips, Michigan Technological University
Rudy Luck, Michigan Technological UniversityFollow
Thomas Werner, Michigan Technological UniversityFollow
Haiying Lui, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

6-14-2019

Abstract

We report a near‐infrared fluorescent probe A for the ratiometric detection of cysteine based on FRET from a coumarin donor to a near‐infrared rhodamine acceptor. Upon addition of cysteine, the coumarin fluorescence increased dramatically up to 18‐fold and the fluorescence of the rhodamine acceptor decreased moderately by 45 % under excitation of the coumarin unit. Probe A has been used to detect cysteine concentration changes in live cells ratiometrically and to visualize fluctuations in cysteine concentrations induced by oxidation stress through treatment with hydrogen peroxide or lipopolysaccharide (LPS). Finally, probe A was successfully applied for the in vivo imaging of Drosophila melanogaster larvae to measure cysteine concentration changes.

Publisher's Statement

© 2019 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. Publisher’s version of record: https://doi.org/10.1002/cbic.201900071

Publication Title

ChemBioChem

Recommended Citation

Xia, S., Zhang, Y., Fang, M., Mikesell, L., Steenwinkel, T., Wan, S., Phillips, T., Luck, R., Werner, T., & Lui, H. (2019). A FRET‐based near‐infrared fluorescent probe for ratiometric detection of cysteine in mitochondria. ChemBioChem, 20, 1-10. http://doi.org/10.1002/cbic.201900071
Retrieved from: https://digitalcommons.mtu.edu/biological-fp/128