Title
A FRET‐based near‐infrared fluorescent probe for ratiometric detection of cysteine in mitochondria
Document Type
Article
Publication Date
6-14-2019
Abstract
We report a near‐infrared fluorescent probe A for the ratiometric detection of cysteine based on FRET from a coumarin donor to a near‐infrared rhodamine acceptor. Upon addition of cysteine, the coumarin fluorescence increased dramatically up to 18‐fold and the fluorescence of the rhodamine acceptor decreased moderately by 45 % under excitation of the coumarin unit. Probe A has been used to detect cysteine concentration changes in live cells ratiometrically and to visualize fluctuations in cysteine concentrations induced by oxidation stress through treatment with hydrogen peroxide or lipopolysaccharide (LPS). Finally, probe A was successfully applied for the in vivo imaging of Drosophila melanogaster larvae to measure cysteine concentration changes.
Publication Title
ChemBioChem
Recommended Citation
Xia, S.,
Zhang, Y.,
Fang, M.,
Mikesell, L.,
Steenwinkel, T.,
Wan, S.,
Phillips, T.,
Luck, R.,
Werner, T.,
&
Lui, H.
(2019).
A FRET‐based near‐infrared fluorescent probe for ratiometric detection of cysteine in mitochondria.
ChemBioChem,
20, 1-10.
http://doi.org/10.1002/cbic.201900071
Retrieved from: https://digitalcommons.mtu.edu/biological-fp/128
Publisher's Statement
© 2019 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. Publisher’s version of record: https://doi.org/10.1002/cbic.201900071