A Key Study on Pollen-Specific SFB Genotype and Identification of Novel SFB Alleles from 48 Accessions in Japanese Apricot (Prunus mume Sieb. et Zucc.)

Authors

Daouda Coulibaly, Nanjing Agricultural University
Guofeng Hu, Nanjing Agricultural University
Zhaojun Ni, Nanjing Agricultural University
Kenneth Omondi Ouma, Nanjing Agricultural University
Xiao Huang, Nanjing Agricultural University
Shahid Iqbal, Michigan Technological UniversityFollow
Chengdong Ma, Nanjing Agricultural University
Ting Shi, Nanjing Agricultural University
Faisal Hayat, Nanjing Agricultural University
Benjamin Karikari, University for Development Studies Ghana
Zhihong Gao, Nanjing Agricultural University

Document Type

Article

Publication Date

9-1-2022

Department

College of Forest Resources and Environmental Science

Abstract

Self-incompatibility (SI) is a common strategy to avoid inbreeding and, consequently, keep genetic diversity within a species. In its mechanism, pollen rejection happens in the style when the single multiallelic locus (SFB in prunus species) of the haploid pollen matches one of the S-alleles existing in the diploid pistil. The SFB gene for the pollen S gene has been identified in many Prunus species. However, Japanese apricot is a species with a typical gametophytic self-incompatibility (GSI), and its SFB alleles available are limited, although they are required for studying GSI. Therefore, we used an AS-PCR amplification method, sequencing, and the pair primers SFB-C1F and Pm-Vb designed based on the conserved region of the Prunus SFB gene to identify SFB genotypes of 48 Japanese apricot (P. mume) accessions. Eleven novel SFB alleles were isolated from these accessions and shared typical structural features with SFB alleles from other Prunus species. These novel SFB alleles were uniquely expressed in pollen. Hence, we concluded that these 11 PmSFB were pollen S determinants of P. mume. This current study offers the novel SFB genes of the P. mume S locus, which could be a useful potential resource for studies on pollen SI mechanisms.

Publisher's Statement

© 2022 by the authors. Licensee MDPI, Basel, Switzerland. Publisher’s version of record: https://doi.org/10.3390/f13091388

Publication Title

Forests

Recommended Citation

Coulibaly, D., Hu, G., Ni, Z., Ouma, K., Huang, X., Iqbal, S., Ma, C., Shi, T., Hayat, F., Karikari, B., & Gao, Z. (2022). A Key Study on Pollen-Specific SFB Genotype and Identification of Novel SFB Alleles from 48 Accessions in Japanese Apricot (Prunus mume Sieb. et Zucc.). Forests, 13(9). http://doi.org/10.3390/f13091388
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/16500

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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Publisher's PDF