Argyreia nervosa-driven biosynthesis of Cu-Ag bimetallic nanoparticles from plant leaves extract unveils enhanced antibacterial properties

Authors

Parvathalu Kalakonda, Department of Physics, Government City College, (A), Nayapul, Osmania University, Hyderabad, Telangana, 500002, India. parvathalu.k@gmail.com.
Rajitha Kathi, Department of Physics, Government City College, (A), Nayapul, Osmania University, Hyderabad, Telangana, 500002, India.
Merlinsheeba Gali Ligory, Department of Biochemistry, University of Hyderabad, Hyderabad, Telangana, 500046, India.
Naveenkumar Dabbeta, Department of Physics, Government City College, (A), Nayapul, Osmania University, Hyderabad, Telangana, 500002, India.
Naveenkumar Madipoju, Department of Physics, Government City College, (A), Nayapul, Osmania University, Hyderabad, Telangana, 500002, India.
Soujanyalakshmi Mynepally, Department of Physics, Government City College, (A), Nayapul, Osmania University, Hyderabad, Telangana, 500002, India.
Vijay Morampudi, Department of Biochemistry, University of Hyderabad, Hyderabad, Telangana, 500046, India.
Sreenivas Banne, Department of Chemistry and Biosciences, Rice University-BRC, Houston, TX, 77005, USA.
Pritam Mandal, Department of Physics, Michigan Technological University, Houghton, MI, 49931, USA.
Ramu Naidu Savu, Department of Chemistry, University of Hyderabad, Hyderabad, Telangana, 500046, India.
Sarvani Jowhar Khanam, Department of Chemistry, University of Hyderabad, Hyderabad, Telangana, 500046, India.
Murali Banavoth, Department of Chemistry, University of Hyderabad, Hyderabad, Telangana, 500046, India.
Naina Vinodini Sudarsanam Eve, Department of Physics, Government City College, (A), Nayapul, Osmania University, Hyderabad, Telangana, 500002, India.
Bala Bhaskar Podila, Department of Physics, Government City College, (A), Nayapul, Osmania University, Hyderabad, Telangana, 500002, India.

Document Type

Article

Publication Date

5-3-2024

Abstract

Our study specifically explores the biosynthesis of copper-silver bimetallic nanoparticles (Cu-Ag BMNPs) using Argyreia nervosa (AN) plant leaf green extract as a versatile agent for capping, reducing, and stabilizing. This biosynthesis method is characterized by its simplicity and cost-effectiveness, utilizing silver nitrate (AgNO) and cupric oxide (CuO) as precursor materials. Our comprehensive characterization of the Cu-Ag BMNPs, employing techniques such as X-ray diffraction (XRD), UV-Vis spectrometry, scanning electron microscopy (SEM), Zetasizer, and Fourier transformed infrared spectrometry (FTIR). FTIR analysis reveals biofunctional groups and chemical bands, while SEM and XRD analyses provide morphological and structural details. To evaluate the antimicrobial properties of the Cu-Ag BMNPs, we conducted disc diffusion and minimum inhibitory concentration (MIC) assays against Escherichia coli (E. coli), with results compared to the standard gentamicin antibiotic. It is observed that the 2% and 5% CuO concentrations of AN Cu-Ag BMNPs exhibit substantial antibacterial activity in comparison to AN extract when tested on EPEC. Among these, the Cu-Ag BMNPs at a 2% concentration demonstrate higher antibacterial activity, potentially attributed to the enhanced dispersion of BMNPs facilitated by the lower CuO doping concentration. These two assays showcased the improved antimicrobial activity of Cu-Ag BMNPs, highlighting their synergistic effect, characterized by high MIC values and a broad zone of inhibition in the disc diffusion tests against E. coli. These results emphasize the significant antibacterial potential of the synthesized BMNPs, with a medicinal plant AN leaf extract playing a pivotal role in enhancing antibacterial activity.

Publication Title

Bioprocess and biosystems engineering

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