Metagenomic analysis of a glacial ice core record from the contiguous United States
Document Type
Article
Publication Date
9-19-2024
Department
Department of Biological Sciences
Abstract
Glacial ice preserves time-sequenced records of preserved microbes, offering access to historic pre-anthropic metagenomes. As proof-of-concept, we three tested methods to recover ancient DNA from a ~250-year ice core from Wyoming's Upper Fremont Glacier for metagenomic sequencing. Direct amplification of filter-concentrated melt water (eDNA) was a simple and effective method for metagenomics. We observed higher microbial diversity, enriched nitrogen-cycling genes, and higher abundance of Nitrosopira, Rhodanobacter and Polaromonas sequences in 1790 CE samples compared to 1900-1961 CE samples. The documented microbial variation may be attributable to changes in climate and land use over the last two centuries.
Publication Title
Research Square
Recommended Citation
Kvitko, B.,
Wallace, J.,
Desai, H. V.,
Lavender, H. F.,
Mijatovic, J.,
Techtmann, S.,
Christner, B. C.,
&
Smith, A.
(2024).
Metagenomic analysis of a glacial ice core record from the contiguous United States.
Research Square.
http://doi.org/10.21203/rs.3.rs-5045654/v1
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p2/1671
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Version
Preprint
Publisher's Statement
© 2024. Publisher’s version of record: https://doi.org/10.21203/rs.3.rs-5045654/v1