Facile enzymic synthesis of caffeoyl CoA
Document Type
Article
Publication Date
2-1-1997
Abstract
p-Hydroxycinnamic acid:CoA ligase (EC 6.2.1.12) was extracted from aspen xylem and its activity with several cinnamic acids was determined. The highest activity was found with p-hydroxycinnamic acid and the extract had reasonable activity with ferulic, caffeic and 5-hydroxyferulic acids but none with sinapic acid. Using this isolated enzyme preparation, caffeoyl CoA was synthesized and subsequently purified using reversed phase C-18 solid phase extraction. This enzymic synthesis method, which yielded 40% product, is rapid and simple and provides a cost-effective alternative to the use of commercial acyl CoA ligase.
Publication Title
Phytochemistry
Recommended Citation
Meng, H.,
&
Campbell, W.
(1997).
Facile enzymic synthesis of caffeoyl CoA.
Phytochemistry,
44(4), 605-608.
http://doi.org/10.1016/S0031-9422(96)00594-8
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/7338