Facile enzymic synthesis of caffeoyl CoA
p-Hydroxycinnamic acid:CoA ligase (EC 18.104.22.168) was extracted from aspen xylem and its activity with several cinnamic acids was determined. The highest activity was found with p-hydroxycinnamic acid and the extract had reasonable activity with ferulic, caffeic and 5-hydroxyferulic acids but none with sinapic acid. Using this isolated enzyme preparation, caffeoyl CoA was synthesized and subsequently purified using reversed phase C-18 solid phase extraction. This enzymic synthesis method, which yielded 40% product, is rapid and simple and provides a cost-effective alternative to the use of commercial acyl CoA ligase.
Facile enzymic synthesis of caffeoyl CoA.
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