Early biosignature of oxidative stress in the retinal pigment epithelium
Document Type
Article
Publication Date
2-1-2011
Abstract
The retinal pigment epithelium (RPE) is essential for retinoid recycling and phagocytosis of photoreceptors. Understanding of proteome changes that mediate oxidative stress-induced degeneration of RPE cells may provide further insight into the molecular mechanisms of retinal diseases. In the current study, comparative proteomics has been applied to investigate global changes of RPE proteins under oxidative stress. Proteomic techniques, including 2D SDS-PAGE, differential gel electrophoresis (DIGE), and tandem time-of-flight (TOF-TOF) mass spectrometry, were used to identify early protein markers of oxidative stress in the RPE. Two biological models of RPE cells revealed several differentially expressed proteins that are involved in key cellular processes such as energy metabolism, protein folding, redox homeostasis, cell differentiation, and retinoid metabolism. Our results provide a new perspective on early signaling molecules of redox imbalance in the RPE and putative therapeutic target proteins of RPE diseases caused by oxidative stress. © 2010 Elsevier B.V.
Publication Title
Journal of Proteomics
Recommended Citation
Arnouk, H.,
Lee, H.,
Zhang, R.,
Chung, H.,
Hunt, R.,
&
Jahng, W.
(2011).
Early biosignature of oxidative stress in the retinal pigment epithelium.
Journal of Proteomics,
74(2), 254-261.
http://doi.org/10.1016/j.jprot.2010.11.004
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/6819