Determination of intracellular pH using sensitive, clickable fluorescent probes
Document Type
Article
Publication Date
4-1-2012
Abstract
We synthesized and evaluated a series of acidic fluorescent pH probes exhibiting robust pH dependence, high sensitivity and photostability, and excellent cell membrane permeability. Titration analyses indicated that probe 3 could increase its fluorescence intensity 800-fold between pH 8.0 and 4.1. Additionally, its pK a value is optimal for intracellular probing of acidic organelles. Fluorescent imaging of HepG2 and Hela cells further revealed that probe 3 demonstrates outstanding capacity for monitoring of intracellular [H +] levels. The easily accessible terminal alkyne/azido function groups of these probes offer the possibility of rapidly constructing sensor molecule libraries using 'click' chemistry. © 2011 Elsevier Ltd. All rights reserved.
Publication Title
Bioorganic and Medicinal Chemistry Letters
Recommended Citation
Yapici, N.,
Mandalapu, S.,
Chew, T.,
Khuon, S.,
&
Bi, L.
(2012).
Determination of intracellular pH using sensitive, clickable fluorescent probes.
Bioorganic and Medicinal Chemistry Letters,
22(7), 2440-2443.
http://doi.org/10.1016/j.bmcl.2012.02.021
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/6054