Electrophoresis of the hemoglobin of three animals in the hibernating and non-hibernating state

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Department of Biological Sciences


1. 1. The phenomenon of the red blood cells of the woodchuck (Marmota monax) being resistant to lysis by the cyanmethemoglobin procedure for hemoglobin was observed during the pre-hibernation period. 2. 2. Electrophoresis was performed on the hemoglobin obtained from these cells in order to determine if a different type of hemoglobin was present during the hibernating period as compared to the non-hibernating period. There was no difference. 3. 3. Hemoglobin samples were also obtained from the little brown bat (Myotis lucifigus) and the golden mantle ground squirrel (Citellus lateralis) during the hibernating period and the non-hibernating period for comparative purposes. All samples were compared to human hemoglobin. 4. 4. There was no difference between the hibernating and non-hibernating pattern .

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Comparative Biochemistry and Physiology -- Part A: Physiology