A modified immunoblot method to identify substrates of protein kinases
Document Type
Article
Publication Date
6-30-2011
Department
Department of Biological Sciences
Abstract
While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis.
Publication Title
Journal of Microbiology
Recommended Citation
Kang, C.,
Jahng, W.,
Husson, R.,
&
Lee, S.
(2011).
A modified immunoblot method to identify substrates of protein kinases.
Journal of Microbiology,
49(3), 499-501.
http://doi.org/10.1007/s12275-011-0465-y
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/5132