Title

A modified immunoblot method to identify substrates of protein kinases

Document Type

Article

Publication Date

6-1-2011

Abstract

While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis. © 2011 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.

Publication Title

Journal of Microbiology

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