Title
A modified immunoblot method to identify substrates of protein kinases
Document Type
Article
Publication Date
6-1-2011
Abstract
While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis. © 2011 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.
Publication Title
Journal of Microbiology
Recommended Citation
Kang, C.,
Jahng, W.,
Husson, R.,
&
Lee, S.
(2011).
A modified immunoblot method to identify substrates of protein kinases.
Journal of Microbiology,
49(3), 499-501.
http://doi.org/10.1007/s12275-011-0465-y
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/5132