Title

A modified T-vector for simplified assembly of hairpin RNAi constructs

Document Type

Article

Publication Date

7-1-2008

Abstract

We describe a modified T-vector, pGFPm-T, for direct cloning of RT-PCR products to generate bidirectional restriction fragments for assembly of hairpin-containing RNAi vectors in the popular pFGC and pGSA binary vector backbone. Green fluorescence protein (GFP) is used as a visual reporter for direct selection of recombinants under UV illumination. The simplified cloning process enables a seamless workflow from candidate gene selection and RT-PCR verification to inverted repeat cloning, using a single pair of gene-specific primers. © 2008 Springer Science+Business Media B.V.

Publication Title

Biotechnology Letters

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