A modified T-vector for simplified assembly of hairpin RNAi constructs
Document Type
Article
Publication Date
7-2008
Department
College of Forest Resources and Environmental Science
Abstract
We describe a modified T-vector, pGFPm-T, for direct cloning of RT-PCR products to generate bidirectional restriction fragments for assembly of hairpin-containing RNAi vectors in the popular pFGC and pGSA binary vector backbone. Green fluorescence protein (GFP) is used as a visual reporter for direct selection of recombinants under UV illumination. The simplified cloning process enables a seamless workflow from candidate gene selection and RT-PCR verification to inverted repeat cloning, using a single pair of gene-specific primers.
Publication Title
Biotechnology Letters
Recommended Citation
Luo, K.,
Harding, S.,
&
Tsai, C.
(2008).
A modified T-vector for simplified assembly of hairpin RNAi constructs.
Biotechnology Letters,
30(7), 1271-1274.
http://doi.org/10.1007/s10529-008-9673-x
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/4838