Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of pseudomonas oleovorans ATCC 8062T
Isolate RS1T isolated from used metalworking fluid was found to be a Gram-negative, motile, and nonspore forming rod. Based on phylogenetic analyses with 16S rRNA, isolate RS1T was placed into the mendocina sublineage of Pseudomonas. The major whole cell fatty acids were C18:1ω7c (32.6%), C16:0 (25.5%), and C15:0 ISO 2OH/C16:1ω7c (14.4%). The sequence similarities of isolate RS1T based on gyrB and rpoD genes were 98.9 and 98.0% with Pseudomonas pseudoalcaligenes, and 98.5 and 98.1% with Pseudomonas oleovorans, respectively. The ribotyping pattern showed a 0.60 similarity with P. oleovorans ATCC 8062T and 0.63 with P. pseudoalcaligenes ATCC17440T. The DNA G + C content of isolate RS1T was 62.2 mol.%. The DNA DNA relatedness was 73.0% with P. oleovorans ATCC 8062T and 79.1% with P. pseudoalcaligenes ATCC 17440T. On the basis of morphological, biochemical, and molecular studies, isolate RS1T is considered to represent a new subspecies of P. oleovorans. Furthermore, based on the DNA DNA relatedness ( < 70%), chemotaxonomic, and molecular profile, P. pseudoalcaligenes ATCC 17440T and P. oleovorans ATCC 8062T should be united under the same name; according to the rules of priority, P. oleovorans, the first described species, is the earlier synonym and P. pseudoalcaligenes is the later synonym. As a consequence, the division of the species P. oleovorans into two novel subspecies is proposed: P. oleovorans subsp. oleovorans subsp. nov. (type strain ATCC 8062T = DSM 1045T = NCIB 6576T), P. oleovorans subsp. lubricantis subsp. nov. (type strain RS1T = ATCC BAA-1494T = DSM 21016T). © Springer Science+Business Media, LLC 2009.
Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of pseudomonas oleovorans ATCC 8062T.
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