Pseudomonas oleovorans subsp. lubricantis subsp. nov., and reclassification of pseudomonas pseudoalcaligenes ATCC 17440T as later synonym of pseudomonas oleovorans ATCC 8062T

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Isolate RS1T isolated from used metalworking fluid was found to be a Gram-negative, motile, and nonspore forming rod. Based on phylogenetic analyses with 16S rRNA, isolate RS1T was placed into the mendocina sublineage of Pseudomonas. The major whole cell fatty acids were C18:1ω7c (32.6%), C16:0 (25.5%), and C15:0 ISO 2OH/C16:1ω7c (14.4%). The sequence similarities of isolate RS1T based on gyrB and rpoD genes were 98.9 and 98.0% with Pseudomonas pseudoalcaligenes, and 98.5 and 98.1% with Pseudomonas oleovorans, respectively. The ribotyping pattern showed a 0.60 similarity with P. oleovorans ATCC 8062T and 0.63 with P. pseudoalcaligenes ATCC17440T. The DNA G + C content of isolate RS1T was 62.2 mol.%. The DNA DNA relatedness was 73.0% with P. oleovorans ATCC 8062T and 79.1% with P. pseudoalcaligenes ATCC 17440T. On the basis of morphological, biochemical, and molecular studies, isolate RS1T is considered to represent a new subspecies of P. oleovorans. Furthermore, based on the DNA DNA relatedness ( < 70%), chemotaxonomic, and molecular profile, P. pseudoalcaligenes ATCC 17440T and P. oleovorans ATCC 8062T should be united under the same name; according to the rules of priority, P. oleovorans, the first described species, is the earlier synonym and P. pseudoalcaligenes is the later synonym. As a consequence, the division of the species P. oleovorans into two novel subspecies is proposed: P. oleovorans subsp. oleovorans subsp. nov. (type strain ATCC 8062T = DSM 1045T = NCIB 6576T), P. oleovorans subsp. lubricantis subsp. nov. (type strain RS1T = ATCC BAA-1494T = DSM 21016T). © Springer Science+Business Media, LLC 2009.

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Current Microbiology