Occurrence and levels of ecdysteroids in spinach
Department of Biological Sciences
The dominant ecdysteroid in spinach, Spinacia oleracea L., is 20-hydroxyecdysone (2β,3β,14α, 20R,22R,25-hexahydroxy-5β-cholest-7-en-6-one) with the presence of a smaller amount of polypodine B (2β,3β,5β,14α,20R,22R,25-heptahydroxycholest-7-en-6-one). Ecdysteroids are present in the seed embryo at 14 μg ecdysteroid/seed, and ecdysteroid levels increase, in the plant during growth and development. During the onset of ecdysteroid production, the ratio of ecdysteroid to total sterol shifts from a value of one to about ten over the course of a week. Spinach may serve as a model to study ecdysteroid biosynthesis in plants.
Occurrence and levels of ecdysteroids in spinach.
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