High-Throughput screening of substrate chemistry for embryonic stem cell attachment, expansion, and maintaining pluripotency
Document Type
Article
Publication Date
2-1-2013
Department
Department of Chemical Engineering
Abstract
Appropriate surface attachment is essential for growing embryonic stem (ES) cells in an undifferentiated state. It is challenging to identify the optimal surface chemistry of the substrata for ES cell attachment and maintenance. Using a rapid, high-throughput polymerization and screening platform with a comprehensive library of 66 monomer-grafted membrane surfaces, the optimal substrate, N-[3-(dimethylamino)propyl] methacrylamide (DMAPMA) has been identified to support strong attachment, high expansion capacity, and long-term self-renewal of ES cells (up to 7 passages). This monomer-based, chemically defined, scalable, sustainable, relatively inexpensive, covalently grafted, and controllable polymeric substrate provides a new opportunity to manipulate surface chemistry for pluripotent stem culture.
Publication Title
Macromolecular Bioscience
Recommended Citation
Zonca, M.,
Yune, P.,
Heldt, C.,
Belfort, G.,
&
Xie, Y.
(2013).
High-Throughput screening of substrate chemistry for embryonic stem cell attachment, expansion, and maintaining pluripotency.
Macromolecular Bioscience,
13(2), 177-190.
http://doi.org/10.1002/mabi.201200315
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/3762
Publisher's Statement
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Publisher’s version of record: https://doi.org/10.1002/mabi.201200315