Detection and quantification of vitamins in microliter volumes of biological samples by LC-MS for clinical screening
Document Type
Article
Publication Date
10-2018
Department
Department of Chemistry; Department of Chemical Engineering
Abstract
A method for simultaneous determination of water-soluble vitamins B1, B2, B3 (nicotinamide), B5, B6 (pyridoxamine), B9 and fat-soluble vitamins E (α-tocopherol) and K1 in tears, and B1, B2, B3, B5, B6, B9, A (retinol), and E in blood serum is described. Liquid chromatography-mass spectrometry (LC-MS) was used with a ternary mobile phase of water and acetonitrile containing 0.1% formic acid and methanol containing 5 mM ammonium formate. Vitamins were quantified using an internal standard method. Using 25 μL injection volumes, the limits of detection were in the range of 0.066-5.3 ng in tear, and 0.087-1.1 ng in serum with linear responses for all vitamins. Intra- and inter-day precision and recoveries were satisfactory. This is the first study to demonstrate simultaneous vitamin detections in microliters of biological samples which has distinct advantages in many diagnostic applications with limited available fluids (e.g., tears; elderly anemic blood) or sampling small subjects (e.g., rodents).
Publication Title
AIChE Journal
Recommended Citation
Khaksari, M.,
Mazzoleni, L. R.,
Ruan, C.,
Song, P.,
Hershey, N.,
Kennedy, R.,
Burns, M.,
&
Minerick, A.
(2018).
Detection and quantification of vitamins in microliter volumes of biological samples by LC-MS for clinical screening.
AIChE Journal,
64(10), 3709-3718.
http://doi.org/10.1002/aic.16345
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/3469