Document Type
Article
Publication Date
4-1-2017
Department
Department of Biological Sciences
Abstract
Vetiver grass is an ideal plant for 2,4,6-trinitrotoluene (TNT) phytoremediation, due to its ability to tolerate and metabolize TNT as previously reported. The current study is the first attempt to investigate the changes in the proteomic profile of a plant under TNT stress. Vetiver plants were grown in nutrient media with varying concentrations of TNT (0, 25, 50, and 100 mg L−1) for 10 days. Although the plants appeared healthy, significant biomass reductions (p = 0.0008) were observed in treated plants. Total proteins in the root decreased significantly (p = 0.0003). Proteomic analysis of root proteins revealed the downregulation of functional proteins involved in key cellular mechanisms such as transcription, ribosome biogenesis, nucleo-cytoplasmic transport of proteins, protein glycosylation, and translation. Growth-related proteins were downregulated; plant defense proteins were upregulated at lower TNT concentrations but downregulated at higher concentrations. Comprehensive understanding of changes in the proteomic profile provides important clues to the mechanism of TNT stress response and tolerance in vetiver.
Publication Title
GeoHealth
Recommended Citation
Das, P.,
Sarkar, D.,
&
Datta, R.
(2017).
Proteomic profiling of vetiver grass (Chrysopogon zizanioides) under 2,4,6-trinitrotoluene (TNT) stress.
GeoHealth,
1(2), 66-74.
http://doi.org/10.1002/2017GH000063
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/2491
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Version
Publisher's PDF
Publisher's Statement
©2017. The Authors. Publisher’s version of record: https://doi.org/10.1002/2017GH000063