Determination of Optical Density (OD) of Oligodeoxynucleotide from HPLC Peak Area

Authors

Komal Chillar, Michigan Technological UniversityFollow
Yipeng Yin, Michigan Technological University
Dhananjani Eriyagama, Michigan Technological UniversityFollow
Shiyue Fang, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

3-15-2022

Department

Department of Chemistry

Abstract

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD260). Here, we describe a method for direct determination of OD260 of ODNs using the area of the peaks in HPLC profiles.

Publisher's Statement

©2022. Publisher’s version of record: https://doi.org/10.26434/chemrxiv-2022-hdqzr

Publication Title

Analytical Chemistry

Recommended Citation

Chillar, K., Yin, Y., Eriyagama, D., & Fang, S. (2022). Determination of Optical Density (OD) of Oligodeoxynucleotide from HPLC Peak Area. Analytical Chemistry, 4. http://doi.org/10.26434/chemrxiv-2022-hdqzr
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/16275

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Version

Publisher's PDF