Title

Determination of optical density (OD) of oligodeoxynucleotide from HPLC peak area

Document Type

Article

Publication Date

1-1-2022

Abstract

Oligodeoxynucleotides (ODNs) are typically purified and analysed with HPLC equipped with a UV-Vis detector. Quantities of ODNs are usually determined using a UV-Vis spectrometer separately after HPLC, and are reported as optical density at 260 nm (OD). Here, we describe a method for direct determination of OD of ODNs using the area of the peaks in HPLC profiles. It is expected that the method will save significant time for researchers in the area of nucleic acid research, and minimize the loss of oligonucleotide samples.

Publication Title

PeerJ analytical chemistry

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