The Dual Specific Hemolysin-X is a Bioactive Natural Product that Forms Pores on the Surface of Erythrocytes
Document Type
Article
Publication Date
4-1-2019
Department
Department of Chemistry
Abstract
Hemolysins are natural cytotoxic agents that are capable of destabilizing plasma membrane structures of erythrocytes. We recently identified a plant hemolysin named Hemolysin-X (HelyX) that showed dual specificity for cholesterol and certain serum glycoproteins, such as asialofetuin and fibrinogen. HelyX was isolated through two different approaches: protein purification protocols as well as organic solvent extraction. Ligand binding properties of HelyX were elucidated by inhibition assays using microtiter plates as well as spectrophotometer. HelyX demonstrated significantly fast kinetics (30–180 seconds) of lysis and ligand binding. Scanning electron microscopic imaging captured distinct pores on the surface of HelyX-treated erythrocytes. Our results indicate that HelyX disintegrates mammalian red blood cells by forming pores on their surfaces.
Publication Title
Biochemistry and Molecular Biology
Recommended Citation
Dam, T. K.,
Welch, C.,
Edwards, J.,
Kadav, P. D.,
&
Hromada, M.
(2019).
The Dual Specific Hemolysin-X is a Bioactive Natural Product that Forms Pores on the Surface of Erythrocytes.
Biochemistry and Molecular Biology,
33(S1), 798.5-798.5.
http://doi.org/10.1096/fasebj.2019.33.1_supplement.798.5
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/15801