Black Dots: Microcontact-Printed, Reference-Free Traction Force Microscopy
Department of Biomedical Engineering
Measuring the traction forces produced by cells provides insight into their behavior and physiological function. Here, we developed a technique (dubbed ‘black dots’) that microcontact prints a fluorescent micropattern onto a flexible substrate to measure cellular traction forces without constraining cell shape or needing to detach the cells. To demonstrate our technique, we assessed human platelets, which can generate a large range of forces within a population. We find platelets that exert more force have more spread area, are more circular, and have more uniformly distributed F-actin filaments. As a result of the high yield of data obtainable by this technique, we were able to evaluate multivariate mixed effects models with interaction terms and conduct a clustering analysis to identify clusters within our data. These statistical techniques demonstrated a complex relationship between spread area, circularity, F-actin dispersion, and platelet force, including cooperative effects that significantly associate with platelet traction forces.
Beussman, K. M.,
Mollica, M. Y.,
Han, S. J.,
Black Dots: Microcontact-Printed, Reference-Free Traction Force Microscopy.
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/15431