Department of Biological Sciences
RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.
Methods and Protocols
Shittu, M. O.,
Rna in situ hybridization for detecting gene expression patterns in the abdomens and wings of drosophila species.
Methods and Protocols,
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/14850
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.