Denaturing reversed-phase HPLC using a mobile phase containing urea for oligodeoxynucleotide analysis
Denaturing reversed-phase (RP) high performance liquid chromatography (HPLC) is usually achieved by elevating column temperature. In this article, an alternative method involving using a mobile phase that contains urea and performing HPLC at room temperature is described. The efficacy of the new method was demonstrated by analyzing a 61-mer oligodeoxynucleotide (ODN) and double-stranded (ds) ODNs. The multiple peaks of the 61-mer ODN under normal conditions merged into one under the denaturing conditions. The broad single peaks of dsODNs under normal conditions were split into two sharp peaks.
Nucleosides, Nucleotides and Nucleic Acids
Denaturing reversed-phase HPLC using a mobile phase containing urea for oligodeoxynucleotide analysis.
Nucleosides, Nucleotides and Nucleic Acids,
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