Dataset Title: 
Intelligen SuperPro Designer Files for Economic Simulation of Batch and Continuous Aqueous Two-Phase Purification for Viral Products

Name and contact information of PI: 
Caryn Heldt
Michigan Technological University
1400 Townsend Drive, Houghton, MI 49931
heldt@mtu.edu
ORCiD: 0000-0002-0776-8763

Funding source
NSF (CBET-1818906) and the Cottrell Foundation

Project summary, description or abstract:
Vaccine manufacturing strategies that lower capital and production costs could improve vaccine access by reducing the cost per dose and encouraging localized manufacturing. Continuous processing is increasingly utilized to drive lower costs in biological manufacturing by requiring fewer capital and operating resources. Aqueous two-phase systems (ATPS) are a liquid-liquid extraction technique that enables continuous processing for viral vectors. To date, no economic comparison between viral vector purifications using traditional methods and ATPS has been published. In this work, economic simulations of traditional chromatography-based virus manufacturing were compared to simulations of ATPS-based virus manufacturing for the same product output in both batch and continuous modes. First, the modeling strategy was validated by re-creating a viral subunit manufacturing economic simulation. Then, ATPS capital and operating costs were compared to that of a traditional chromatography purification at multiple scales of production. At all scales, ATPS purification required less than 10% of the capital expenditure compared to traditional chromatography-based purification. The production cost differences increased as manufacturing scale increased and were mainly attributed to chromatography resins required for the traditional method and absent from ATPS. At 11 kg per year production, the largest scale explored, the ATPS process cost 50% less than purification with chromatography per kg of viral product created. Based on these economic models, batch and continuous ATPS were similar in capital and production costs. These simulations show the significant reduction in manufacturing costs that ATPS-based purification could deliver to the vaccine industry.

Brief description of collection and processing of data:
SuperPro Designer files were created to model two externally published purification processes and two purification processes originating from the Heldt Bioseparations lab. 

Description of files:
* Nold Chuan Validation 500L Fermenter SuperPro.spf
o Model of virus subunit purification detailed by Chuan et al. This was used as the validation model in Nold, N. M. et al. 
* Nold Kalbfuss Traditional Chromatography SuperPro.spf
o Model of virus purification by size exclusion chromatography and ion exchange chromatography in batch mode detailed by Kalbfuss et al. Only the process with 11 kg output has been included. 
* Nold Batch ATPS SuperPro 11kg.spf
o Model of virus purification by aqueous two-phase system (ATPS) in batch mode. Only the process with 11 kg output has been included. 
* Nold Continuous ATPS SuperPro 11kg.spf
o Model of virus purification by aqueous two-phase system (ATPS) in continuous mode. Only the process with 11 kg output has been included. 

Definition of acronyms, codes, and abbreviations:
ATPS: Aqueous Two-Phase Systems
SEC: Size Exclusion Chromatography
AEX: Anion Exchange Chromatography
PEG: Polyethylene glycol

Temporal (beginning and end dates of data collection)
Files created using versions 10 and 12 of IntelligenÕs SuperPro Designer. 2019 dollar were used to simulate capital and operating costs for all processes. 

Special software required to use data:
Intelligen SuperPro Designer versions 10 and up

Publications that cite or use this data:
Nold, N. M., Pearson, E., and Heldt, C. L. Submitted. 

Was data derived from another data source?Ê If so, what source?
* Chuan, Y.P., et al., The economics of virus-like particle and capsomere vaccines. Biochemical Engineering Journal, 2014. 90: p. 255-263.
* Kalbfuss, B., et al., Purification of cell culture-derived human influenza A virus by size-exclusion and anion-exchange chromatography. Biotechnol Bioeng, 2007. 96(5): p. 932-44.
* Joshi, P.U., et al., Tie line framework to optimize non-enveloped virus recovery in aqueous two-phase systems. J Chromatogr B Analyt Technol Biomed Life Sci, 2019. 1126-1127: p. 121744.
* Turpeinen, D.G., et al., Continuous purification of an enveloped and non-enveloped viral particle using an aqueous two-phase system. Separation and Purification Technology, 2021. 269.