Antibacterial properties of mussel-inspired polydopamine coatings prepared by a simple two-step shaking-assisted method

Authors

Pegah Kord Fooroshani, Michigan Technological UniversityFollow
Elizabeth Polega, Michigan Technological UniversityFollow
Kevin Thomson, Michigan Technological UniversityFollow
Mohammad Saleh Akram Bhuiyan, Michigan Technological UniversityFollow
Rattapol Pinnaratip, Michigan Technological UniversityFollow
Mikhail Trought, Michigan Technological UniversityFollow
Chito E. Kendrick, Michigan Technological UniversityFollow
Yuesheng Gao, Michigan Technological UniversityFollow
Kahryn Perrine, Michigan Technological UniversityFollow
Lei Pan, Michigan Technological University
Bruce P. Lee, Michigan Technological UniversityFollow

Document Type

Article

Publication Date

9-25-2019

Department

Department of Biomedical Engineering; Department of Chemistry; Department of Electrical and Computer Engineering; Department of Chemical Engineering

Abstract

A simple two-step, shaking-assisted polydopamine (PDA) coating technique was used to impart polypropylene (PP) mesh with antimicrobial properties. In this modified method, a relatively large concentration of dopamine (20 mg ml⁻¹) was first used to create a stable PDA primer layer, while the second step utilized a significantly lower concentration of dopamine (2 mg ml⁻¹) to promote the formation and deposition of large aggregates of PDA nanoparticles. Gentle shaking (70 rpm) was employed to increase the deposition of PDA nanoparticle aggregates and the formation of a thicker PDA coating with nano-scaled surface roughness (RMS = 110 nm and Ra = 82 nm). Cyclic voltammetry experiment confirmed that the PDA coating remained redox active, despite extensive oxidative cross-linking. When the PDA-coated mesh was hydrated in phosphate saline buffer (pH 7.4), it was activated to generate 200 μM hydrogen peroxide (H₂O₂) for over 48 h. The sustained release of low doses of H₂O₂ was antibacterial against both gram-positive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria. PDA coating achieved 100% reduction (LRV ~3.15) when incubated against E. coli and 98.9% reduction (LRV ~1.97) against S. epi in 24 h.

Publisher's Statement

© 2019 Kord Forooshani, Polega, Thomson, Bhuiyan, Pinnaratip, Trought, Kendrick, Gao, Perrine, Pan and Lee. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). Publisher’s version of record: https://doi.org/10.3389/fchem.2019.00631

Publication Title

Frontiers in Chemistry

Recommended Citation

Kord Fooroshani, P., Polega, E., Thomson, K., Bhuiyan, M. S., Pinnaratip, R., Trought, M., Kendrick, C. E., Gao, Y., Perrine, K., Pan, L., & Lee, B. P. (2019). Antibacterial properties of mussel-inspired polydopamine coatings prepared by a simple two-step shaking-assisted method. Frontiers in Chemistry, 7, 1-15. http://doi.org/10.3389/fchem.2019.00631
Retrieved from: https://digitalcommons.mtu.edu/michigantech-p/830

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Version

Publisher's PDF