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Date of Award
2015
Document Type
Campus Access Master's Thesis
Degree Name
Master of Science in Biological Sciences (MS)
Administrative Home Department
Department of Biological Sciences
Advisor 1
Ramakrishna Wusirika
Advisor 2
Aparna D. Deshpande
Committee Member 1
Rupali Datta
Committee Member 2
Tarun Dam
Abstract
Rice callus suspension culture (RCSC) was previously shown to have anticancer activity in colorectal and renal cancer cell lines minimal or no effect on normal cell line. In this study, we tested the effect of RCSC by studying the dynamics of lactate dehydrogenase (LDH) and metabolic viability through WST-1 assay in breast, colorectal and lung cancer cell lines as well as their normal-prototypes. Lower dilutions of RCSC showed best effect in lung cancer cell lines with minimal effect on normal cell lines. Fluorescence microscopy with stains targeting nucleus, cell membrane, chromatin and caspase 3/7 suggested apoptosis as the most likely event induced by RCSC. Gene expression analysis identified cJUN, NF-KB2 and ITGA2B genes to be upregulated resulting in arrest of cell cycle progression and induction of apoptotic process. Lastly, HPLC derived RCSC fractions were found less effective in reducing cell viability than whole RCSC suggesting that a holistic approach is more efficient in inhibiting cancer cell proliferation.
Recommended Citation
Rahman, Nafeesa, "Rice callus suspesion culture acts as an efficient anticancer agent on lung cancer cell line inducing apoptosis", Campus Access Master's Thesis, Michigan Technological University, 2015.